Simultaneous Estimation of Nebivolol Hydrochloride and Valsartan using RP HPLC
نویسندگان
چکیده
In this study, a rapid, precise, accurate, specific and sensitive ion-paired reverse phase liquid chromatographic method has been developed for the simultaneous estimation of nebivolol hydrochloride and valsartan in their capsule formulation. The chromatographic method was standardized using a HIQ sil C(18) column (250x4.6 mm i.d., 5 mum particle size) with UV detection at 289 nm and flow rate of 1 ml/min. The mobile phase consisting of methanol:water (80:20 v/v) with addition of 0.1 percent 1-hexanesulfonic acid monohydrate sodium salt as an ion-pairing reagent was selected. The method was validated and produced accurate and precise results for estimation of the two drugs.
منابع مشابه
Simultaneous estimation of amlodipine besylate and nebivolol hydrochloride in pharmaceutical tablets formulation by RP-HPLC using PDA detector
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